連續培養
A+醫學百科 >> 連續培養 |
連續培養又叫開放培養,是相對分批培養或密閉培養而言的。連續培養是采用有效的措施讓微生物在某特定的環境中保持旺盛生長狀態的培養方法.微生物在一定條件下,如果不斷補充營養物質和排除有害的代謝產物,理論上,微生物都可保持恒定的對數生長.通常采用兩種方式:(1)恒濁培養,當微生物在恒濁器中培養進入對數期時,不斷從外界加入新鮮培養基,而同時又流出培養物,用光電池信號控制濁度,以維持恒定的細胞密度.(2)恒化培養,微生物在恒化器中培養,通過控制恒化器中微生物生長所必需營養物的濃度,以保證微生物持續生長.連續培養應用于工業發酵中稱連續發酵,并已實際應用,例如,連續發酵法生產酒精,半連續發酵法生產丙酮、丁醇等.GOOGLE英文翻譯:The adoption of effective measures to allow micro-organisms in a particular environment to maintain strong growth of the state of training methods. Microorganism under certain conditions, if the continuous supply of nutrients and removal of harmful metabolites, in theory, micro-organisms can remain constant growth of the logarithm of . commonly used in two ways: (1) constant turbid culture, when the micro-organisms cultivated at constant turbidity device into the right number of periods, and constantly adding fresh medium from the outside world, while at the same time out of cultures, the battery light signal control turbidity, in order to maintain a constant cell density (2). chemostat cultivation of microorganisms in the chemostat culture, by controlling the growth of microorganisms in chemostat necessary for the concentration of nutrients in order to ensure sustained growth of microorganisms. used in industrial fermentation in continuous culture said the continuous fermentation, and has practical applications, for example, continuous production of fermentation alcohol, semi-continuous fermentation production of acetone, butanol, and so on.
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